A mixture of 30.4 g glycerol, 1.09 ml of water, 1 g of Lipozyme TLIM and 100 g of coconut oil was prepared. The reaction mixture was first incubated at 3O0C for 6 h with constant stirring at 800 r.p.m. The mixture was then transferred to 50C for up to 3 days before analyze.
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