Oxalic cardboard, ethanol gel, oils vaporizer

The trials started on September 15, 2016 and the experimental colonies were treated
weekly over 4 weeks with three formulations.

Treatment 1, intended for slow miticide release, consisted of a 2% oxalic
acid solution dissolved in a syrup made up of 65% sucrose and 35% H2O (w:v). A
corrugated cardboard piece (30 x 23 x 0.5 cm) was soaked with 250 mL of the solution
and placed on top of the brood chamber frames of each hive. Therefore, each colony
received approximately 5 g of oxalic acid as active ingredient with each application.

Treatment 2, intended for rapid miticide release, was a 7% solution of a mixture of oregano oil and clove oil in equal proportions. The solvent was prepared with 15 parts of 96% ethanol, 15 parts of H2O, two parts of food-grade mineral oil and one part of food-grade gelatin.
Then, 30 ml of this solution were used to impregnate each of three 15 x 9 x 0.3 cm
absorbent pads which were applied in hives as above, as recommended by Gashout (2008), who found promising miticidal results for thymol with this delivery system. Each of these three pads contained approximately 1 g of each essential oil.

Treatment 3, intended for continuous miticide release, was oregano oil in the 20 ml containers of two electric vaporizers.

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Make Your Own Gelled Alcohol

1. Add 25g of crushed chalk or egg shells (calcium carbonate-CaCO3) to 100ml of vinegar (water and acetic acid - CH3CO2H) and stir for about 5 minutes.

2. Remove the excess chalk by filtering your mix through some filter paper (coffee filter or napkin can be used).

3. Mark the level of your solution in its container and allow your solution to evaporate off about half that volume to remove the excess water.

4. Add 30ml of alcohol (ethanol, methanol, or isopropanol) to your solution and watch the gel form. Do not stir.