20% sulfur;
30% garlic pulp;
50% ethanol 99 degree;
Add plate or textile pad in hive for evaporation. Contact mode of action.

EXAMPLE

In a 250 ml vessel:
30 g of double ventilated elementary sulphur,
30 g of crushed garlic bulbs,
10 g of dried pepper powder (Capsicum annuum) piquant variety,
30 g of 99% by volume ethanol are mixed.
The mixture is stirred by means of a mechanical stirrer to a thick consistency.

The composition is applied to a hive for breeding Apis mellifica infested by Varroa Jacobsoni, located in nortwest Italy, by covering both the side walls and the bottom and the cover of the hive. The application was made in the month of June. The hive bottom was 30×30 cm. After about three months (at the end of August) the hive was inspected and a number of dead mites between 40,000 and 42,000 was counted on the hive bottom. The hive bottom has been cleaned and the treatment has been repeated using the same composition in the same way as above. After about two months (at the end of October) the hive has been inspected again. About 500 dead mites were counted on the hive bottom. After properly cleaning, the treatment has been applied again with the same composition. After about four months (at the end of February in the following year) the inspection was repeated and a single dead mite was found on the hive bottom. An examination of the bees showed the absence of the parasite.

Sulfur: Flowers of sulphur was tested on 28 285 infested colonies of honeybees. Three methods of application were compared: (1) sprinkling the powder on the frame top-bars at 0.2 g per bee way; (2) rubbing the sulphur into a cloth covering the frames, at 10 g per hive; (3) sprinkling 10 g onto sticky paper on the hive floor. Trials were made in April, July and October, 1975. At all seasons method 2 was the most effective.

Full article